Balaji Tissue Culture Banana Plants - Grand-9 (Polybag)

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 Only Information

Banana Tissue Culture Plants
Variety: Grand-9 (Polybag)

Minimum Order Quantity is 5000 Plants
Plants will be dispatched from Coimbatore

Free Delivery for the States: Kerala, Tamilnadu, Karnataka, Andhra Pradesh and Telangana and Maharashtra only.
For Other States, Transportation will be charged extra.

For technical information or extra transportation cost, contact:
Business Development Manager,
Shri Balaji Agrobiotech,
+91 9483280275

Plant Tissue Culture
Tissue culture is a process of cloning and micro propagation of tissues of the selected quality plants & daughter suckers or Tissue culture is the modern technology that can be applied for mass production of superior grade planting material for most crops. This method of cultivation or production ensures not only higher yields but quality as well. The Tissue culture technology is composed of 5 important steps such as Initiation, Multiplication, Shooting & rooting, Primary hardening in a greenhouse and Secondary hardening in shade net houses. To be successful in Tissue culture method, one should adhere to standards and micro-climatic conditions & care during the hardening process can ensure success.

What is tissue culture?
The propagation of plant by using plant part or single cell in test tube under very controlled and hygienic conditions is called “Tissue Culture”.

                        Banana Plant Tissue Culture Steps
Advantages of Tissue Culture:
There are several benefits of using tissue cultured banana plantlets when compared to regular (conventional) suckers.
  • Banana plants produced from Tissue culture technology are high yielding of true type and can have uniform superior clones of different varieties available in large numbers (quantities)
  • Banana plantlets of the Tissue culture are disease free at the time of planting. Same conditions (disease free) can be maintained by proper crop management practices.
  • Tissue culture plantlets are available throughout the year. So Bananas can be grown year round without any shortage of tissue cultured plant material. Tissue culture plantlets are available throughout the year. So Bananas can be grown year round without any shortage of tissue cultured plant material.
  • The other biggest advantage is that tissue-cultured banana plants produce uniform maturity of the fruits which can make harvesting process easy and reduce labor cost.
  • Ratoon crop (The main benefit of ratooning is that the crop matures earlier in the season) is a unique feature of Tissue culture banana plants.
  • Banana produced from Tissue culture Banana plants gives a better price in the market due to their quality and uniformity.
  • Using tissue culture plants, one can obtain 3 crops in 30 to 32 months period which can save 4 to 5 months of cultivation period in the field.
             Green house facility                            Grang 9 (Polybag)      Grand 9 (Netpot)    

Grand 9 polybags at green house facility

Planting Time
Planting of tissue culture Banana can be done throughout the year except when the temperature is too low or too high. There are two important seasons;
Mrig Baug (Kharif) month of planting June-July
Kande baug (Rabi) month of planting October-November
Land Preparation
Soil should be well prepared with 1 or 2 successive   ploughing and harrowing. Prepare pit of size 1’*1’*1’ at the desired spacing. Mix 2-3 Kg FYM,5 gm prorate and 200 gm neem and 10 gm Furadon cake into quantity of soil and add this mixture into the pit. Then remove T.C Banana plant from polyethylene bag and plant it at the center of the pit.
Spacing and Planting
Spacing generally depends on cultivar/variety and planting method. In the case of Dwarf varieties, it requires spacing of 5.5 feet x 5.5 feet and plant density (Number of plants) required is about 1400 to 1450/acre. Red banana variety requires 8 ft. x 8 ft. and plant density would be around 700/acre. There are other banana varieties like Giant Cavendish, Nendran, Robusta and Grandnaine, Williams requires spacing from 6 feet x 6ft – 7 feet x 7feet and no of plants required per acre are from 900 to 1200. Do not plant too shallow or too deep and allow a space of 10 cm between the plant and the soil surface.

                                  Banana Tissue Culture Plants in the field
The frequency of irrigation depends on soil moisture holding capacity and climate. For proper utilization of water in tissue culture planting, it is recommended to adopt drip irrigation. Irrigate the Banana plantation immediately after planting in the field. There is a government subsidy for drip irrigation as well. Usually, a Banana plant requires 20 to 25 liters of water per day under drip irrigation system. In hot summers or dry spells, it is a good practice to give flood irrigation once in a month apart from using the drip system.
The rate of irrigation water per plant per day.
Month (Maug Baug) Qty. (LPD) Month (Kande baug) Qty. (LPD)
June 06 October 04-06
July 05 November 04
August 06 December 04
September 08 January 06
October 10-12 February 08-10
November 10 March 10-12
December 10 April 16-18
January 10 May 18-20
February 12 June 12
March 16-18 July 12
April 20-22 August 14
May 25-30 September 14-16
Manures and Fertilizers
The schedule of manures and fertilizers in Tissue culture plantation depends on soil fertility. Based on soil test results, appropriate manures and fertilizers should be applied. However, the following manures and fertilizers gave for your reference.
Water Soluble Fertilizers
Use water soluble fertilizer with drip irrigation system. It helps in saving of fertilizer, increases yield and stops deposition of salts because of the acidic nature of the fertilizer. Tablr (2) shows the time and quantity of the fertilizer.
Standard Doses for Fertilizers
Nitrogen-170 gm/plant; Phosphorus-45 gm/plant; Potash-185 gm/plant. Total Fertilizer required for 1 Acre=218 Kg N: 65 Kg P:272 Kg K2o Planting Distance=6’ * 5’ (1452 plants)
Table 2
 Time of Application Grade Total Qty.(kg) Fertilizers/day     
1 to 50 days after 19:19:19 + 192 2.13  
plantation ( micro-nutrient)+ 93 1.033  
  13:0:46 + 70 0.777  
After 75 days Micronutrient + 14.5 10 gm/plant  
  MgSo4 + 36.3 25 gm/plant  
91 to 150 days 0:52:34 + 59 0.983  
  13:0:46 + 59 0.983  
  Urea 59 0.983  
151 to 300 days 13:0:46 + 282.5 1.884  
  Urea 150 1.00  
Traditional Fertilizers=For the traditional fertilizer 200 gm N;60 gm P2O5 250 gm K2O is recommend per    plant.
Table 3  
  Time of Application Grade Total fertilizer Fertilizer
      required for Required
      1000 plants (gm/plant)
  At the time Super phosphate + 125 Kg. 125 gm
  of plantation potash 105 Kg 105 gm
  After 30 days Urea 62 kg. 62 gm
  After 75 days Urea + 62 kg. 62 gm
    Super phosphate + 125 Kg. 125 gm
    micro-nutrients + 25 kg 25 gm
    MgSo4 25 kg 25 gm
  After 125 days Urea + 62 kg. 62 gm
    Super phosphate + 125 Kg. 125 gm
  After 165 days Urea + 62 kg. 62 gm
    Potash 105 kg 105 gm
  After 210 days Urea 62 kg 62 gm
  After 255 days Urea + 62 kg. 62 gm
    Potash 105 kg 105 gm
  After 300 days Urea + 62 kg. 62 gm
    Potash 105 kg 105 gm
*Fertilizer recommendations in Table 2&3 are only a guideline. Please make necessary changes as per soil analysis and experience. Use of WSF is found to be economical.
Intercropping in Bananas is found to be more profitable. Between two rows or two pair rows onion can be planted.
Only at the early stages of banana intercropping is possible.
Inter cultivation
Keep the field free from weed. Keep the soil loose, airy with harrowing. Cut the additional shoots from time to time. Protect the growing bunch by covering it with leaves.
Control of Sigatoka Disease
If any symptom or leaf spot of this disease is observed, please do the following measures.
  1. Remove diseased leaves.
  2. Spray 100 gm of Bavistin,250 gm of Dithane M-45 or copper oxychloride in 100 liters water. Use stickers so that pesticide mixture can stick to leaves.
  3. Use above solution for drenching. Apply 250 ml of the solution for one plant without mixing the sticker.
Ratoon Crop
After 7-9 month from the planting or after 40-50% flowering, one healthy, newly born, sword leaves sucker arising from the main plant should be retain as a Ratoon, and remaining sucker should be cut. The Management practices and fertilizer schedule for ratoon crop is the same as for the main crop.
Yield is approximately double if tissue culture plants are planted with proper cultivation, soil preparation, water and fertilizer management. In some cases, yield rose to 3 times than that of sucker plantation.

            Grand 9                                   Nendran Quinta                                  Red Banana                            
Comparison of Tissue Culture Banana plantation and Traditional Sucker plantation
Tissue Culture Plantation Traditional Sucker plantation
1)   Plants are of same age and variety 1)   It is not sure that all the suckers are of same age and Therefore not uniform.
2)  Plants are disease free and healthy 2)  Some suckers may be diseased.
3)  Plants carry same characters of mother plant 3)  In case of suckers there is no possibility of assuring
4)  As the suckers are selected from the mother plant with 4)  Low yield is observed because suckers are from
high yields the tissue culture plants give high yield** different mother plants having a range of yield level.
5)  Crop is ready for harvest in 11 to 12 months from 5)  Crop is ready for harvest in 15 to 16 months from
plantation. plantation.
6)  Crop growth is uniform. 6)  Crop growth is not uniform.
7)  Costs of irrigation labour and cultivation are less 7)  Costs of irrigation  labor and cultivation are more
because crop period is short** because crop period is long.
8)   In 28-30 months one main crop and two  ratoon crop 8)  In 30-32 month one main crop and only one ratoon
can be harvested** crop are possible.
9)  More yield ,and more profit** 9)  Less yield  less profit.
In India Banana occupies 20% area among the total area under crop. The total annual production is 17.0 Million Tones from around 500 thousand Ha. Now approximately 25% of plantation are done by tissue culture plants.
Considering availability of water, the cultivation of Banana using tissue culture Plants is the most profitable way than using suckers. The profit is almost the double while using the tissue culture plants.
Unlike the suckers tissue culture plants are of same age and verity as well as disease free. Their growth is uniform and are ready for harvest in 11 to 12 months from plantation. The cost of cultivation is less since the crop period is short. After all, the farmers will get more yield and more profit by using tissue culture banana plants. Large volume of plants is available at any season.
At the time of planting Farm yard manure 5 kg
  Single Super Phosphate 300 Grams
  Neem cake 500 Grams
  Furadon 10 Grams
After 35 days Single Super Phosphate 200 Grams
  Muriate of Potash 125 Grams
  Urea 125 Grams
After 75 days Caster Cake 1 kg
  Single Super Phosphate 200 Grams
After 125 days Urea 125 Grams
  Muriate of Potash 175 Grams
After 165 days Neem Cake 1 kg
After 210 days Urea 107 Grams
  Muriate of Potash 150 Grams
Days After Planting Pesticides Dosage
15 Days Monocrotophos 1 ML per Liter
  Bavistin 1 Gram per Liter
45 Days Nuvacron 1 ML per Liter
  Diathane - M45 1 Gram per Liter
75 Days Monocrotophos 1 ML per Liter
  Bavistin 1 Gram per Liter
105 Days uvacron 1 ML per Liter
  Diathane - M45 1 Gram per Liter

             Nandran (Packing Stage)                                 Elekki (Packing Stage)

Dry and dead leaves must be removed and the field must be kept clean. Keep removing side suckers till flowering to allow the mother plant grow healthier and leave one sword sucker at the time of flower emergence. Remove diseased plants and burn. Fertilizer should be added at least one foot away from plant in 5cms. Deep ring and ensure irrigation immediately. Weeding and earthling up operation should be done at 3 to 4 week intervals to keep the soil loose in the first 4 to 5 months after planting. When hands of bunches are opened remaining part of the flower should be removed. During the summer, bunch should be covered with dried and old leaves to protect the bunches from sun scorching.